Home Biology Biotechnology and Its Application Statement Based Questions Some of the steps involved in the production…
Biology Biotechnology and Its Application Statement Based Questions Single Correct MCQ
Published on: August 21, 2026

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.

(1) Purification of humulin.

(2) Extraction of recombinant gene product from E. coli.

(3) Culturing recombinant E. coli in bioreactors.

(4) Introduction of recombinant plasmid into E. coli.

(5) Synthesis of gene for human insulin artificially.

(6) Insertion of human insulin gene into plasmid.

A
(2), (1), (4), (3), (5), (6)
B
(1), (3), (5), (6), (2), (4)
C
(5), (6), (4), (3), (2), (1)
D
(3), (5), (2), (1), (6), (4)

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Text Solution

Verified by Experts
The correct answer is:
C

(5) Synthesis of human insulin gene artificially.

(6) Insertion of insulin gene into plasmid.

(4) Introduction of recombinant plasmid into E. coli.

(3) Culturing recombinant E. coli in bioreactors.

(2) Extraction of recombinant insulin product.

(1) Purification of Humulin (human insulin).

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