Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
(1) Purification of humulin.
(2) Extraction of recombinant gene product from E. coli.
(3) Culturing recombinant E. coli in bioreactors.
(4) Introduction of recombinant plasmid into E. coli.
(5) Synthesis of gene for human insulin artificially.
(6) Insertion of human insulin gene into plasmid.
Text Solution
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(5) Synthesis of human insulin gene artificially.
(6) Insertion of insulin gene into plasmid.
(4) Introduction of recombinant plasmid into E. coli.
(3) Culturing recombinant E. coli in bioreactors.
(2) Extraction of recombinant insulin product.
(1) Purification of Humulin (human insulin).
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